| dc.contributor.author | Sel, Figen Abatay | |
| dc.contributor.author | Erol, Ayşe | |
| dc.contributor.author | Süleymanoğlu, Mediha | |
| dc.contributor.author | Savran Oğuz, Fatma | |
| dc.contributor.author | Kuruca, Dürdane Serap | |
| dc.date.accessioned | 2025-03-13T06:46:02Z | |
| dc.date.available | 2025-03-13T06:46:02Z | |
| dc.date.issued | 2024 | en_US |
| dc.identifier.citation | Abatay Sel, F., Erol, A., Suleymanoglu, M., Kuruca, D. S., & Savran Oguz, F. (2024). Easy and Rapid Methods for Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Human Umbilical Wharton's Jelly-Derived Mesenchymal Stem Cells. Methods in molecular biology (Clifton, N.J.), 2736, 77–84. https://doi.org/10.1007/7651_2023_479 | en_US |
| dc.identifier.uri | https://hdl.handle.net/20.500.12900/521 | |
| dc.description.abstract | These protocols describe modified methods that use Ficoll-Paque density gradient for umbilical cord blood-derived mesenchymal stem cells and explant method for Wharton's jelly-derived mesenchymal stem cells. The Ficoll-Paque density gradient method allows to obtain mesenchymal stem cells while eliminating monocytic cells. In this method, precoating the cell culture flasks with fetal bovine serum helps remove the monocytic cells and instruct more pure mesenchymal stem cells. On the other hand, the explant method for Wharton's jelly-derived mesenchymal stem cell is user-friendly and cost-effective than enzymatic methods. In this chapter, we provide a collection of protocols to obtain mesenchymal stem cells from human umbilical cord blood and Wharton's jelly. | en_US |
| dc.language.iso | eng | en_US |
| dc.publisher | SPRINGER | en_US |
| dc.relation.isversionof | 10.1007/7651_2023_479 | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Explant method | en_US |
| dc.subject | Ficoll-Paque density gradient | en_US |
| dc.subject | Mesenchymal stem cell | en_US |
| dc.subject | Umbilical cord blood | en_US |
| dc.subject | Wharton’s jelly | en_US |
| dc.title | Easy and Rapid Methods for Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells and Human Umbilical Wharton's Jelly-Derived Mesenchymal Stem Cells | en_US |
| dc.type | article | en_US |
| dc.department | İstanbul Atlas Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü | en_US |
| dc.contributor.institutionauthor | Kuruca, Dürdane Serap | |
| dc.relation.journal | Stem Cells and Lineage Commitment | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
