Temel Tıp Bilimleri Bölümü Koleksiyonuhttps://hdl.handle.net/20.500.12900/772024-03-28T14:20:02Z2024-03-28T14:20:02ZThe Association between Serum 25-Hydroxyvitamin D3 Levels and Pro-Inflammatory Markers in New-Onset Type 2 Diabetes Mellitus and PrediabetesFenercioglu, Aysen KutanGonen, Mustafa SaitUzun, HafizeSipahioglu, Nurver TurfanerCan, GunayTas, EbruKara, Zehrahttps://hdl.handle.net/20.500.12900/3252024-03-08T08:48:33Z2023-01-01T00:00:00ZThe Association between Serum 25-Hydroxyvitamin D3 Levels and Pro-Inflammatory Markers in New-Onset Type 2 Diabetes Mellitus and Prediabetes
Fenercioglu, Aysen Kutan; Gonen, Mustafa Sait; Uzun, Hafize; Sipahioglu, Nurver Turfaner; Can, Gunay; Tas, Ebru; Kara, Zehra
In this study, we aimed to reveal the pro-inflammatory effects of serum 25-hydroxyvitamin D3 (Vit D) deficiency and insufficiency in new-onset type 2 diabetes mellitus (T2DM) and prediabetes. We recruited 84 prediabetes patients, 94 new-onset T2DM patients and 113 healthy participants. We measured the levels of C-reactive protein (CRP), fibrinogen, ferritin, interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), nuclear factor kappa-B (NF-kappa B) and mitogen-activated protein kinase (MAPK) in the serum of the participants. ANOVA Bonferroni and Kruskal-Wallis Dunn tests were used to compare the inflammation markers and vitamin D levels between the groups. Based on covariance analysis with age, gender and BMI, the Vit D levels of the T2DM group were significantly lower (p < 0.003). Pro-inflammatory markers and CRP were significantly higher in prediabetic and diabetic subjects. In the prediabetes group, IL-1 beta, IL-6, IL-8, TNF-alpha and MAPK were significantly higher in those with Vit D insufficiency and deficiency groups. In the T2DM group, IL-1 beta, IL-6, IL-8, TNF-alpha, NF-kappa B, MAPK and CRP were significantly higher in those with Vit D insufficiency and deficiency. Our study emphasizes the pro-inflammatory effects of Vit D deficiency and insufficiency in new-onset type 2 diabetes mellitus and prediabetes.
2023-01-01T00:00:00ZPrediction of Preterm Delivery Using Serum Ischemia Modified Albumin, Biglycan, and Decorin Levels in Women with Threatened Preterm LaborBıyık, İsmailSoysal, Cenkİnce, Özlem Ulaş OnurDurmuş, SinemÖztaş, EfserKeskin, NadiIşıklar, Özben ÖzdenKaraağaç, Oğuz HanGelişgen, RemiseUzun, Hafizehttps://hdl.handle.net/20.500.12900/3192024-03-07T06:38:26Z2023-01-01T00:00:00ZPrediction of Preterm Delivery Using Serum Ischemia Modified Albumin, Biglycan, and Decorin Levels in Women with Threatened Preterm Labor
Bıyık, İsmail; Soysal, Cenk; İnce, Özlem Ulaş Onur; Durmuş, Sinem; Öztaş, Efser; Keskin, Nadi; Işıklar, Özben Özden; Karaağaç, Oğuz Han; Gelişgen, Remise; Uzun, Hafize
Objective The serum ischemia modified albumin (IMA), biglycan, and decorin levels of pregnant women who were hospitalized for threatened preterm labor were measured.Methods Fifty-one consecutive pregnant women with a single pregnancy between the 24( th) and 36 (th) weeks with a diagnosis of threatened preterm labor were included in the present prospective cohort study.Results As a result of multivariate logistic regression analysis for predicting preterm delivery within 24 hours, 48 hours, 7 days, 14 days, <= 35 gestational weeks, and <= 37 gestational weeks after admission, area under the curve (AUC) (95% confidence interval [CI[) values were 0.95 (0.89-1.00), 0.93 (0.86-0.99), 0.91 (0.83-0.98), 0.92 (0.85-0.99), 0.82 (0.69-0.96), and 0.89 (0.80-0.98), respectively. In the present study, IMA and biglycan levels were found to be higher and decorin levels lower in women admitted to the hospital with threatened preterm labor and who gave preterm birth within 48 hours compared with those who gave birth after 48 hours.Conclusion In pregnant women admitted to the hospital with threatened preterm labor, the prediction preterm delivery of the combined model created by adding IMA, decorin, and biglycan in addition to the TVS CL measurement was higher than the TVS CL measurement alone.
2023-01-01T00:00:00ZThe Role of Glycogen Synthase Kinase-3β in the Zinc-Mediated Neuroprotective Effect of Metformin in Rats with Glutamate NeurotoxicityOruc, AykutOruc, Kadriye YagmurYanar, KarolinMengi, MuratCaglar, AyselKurt, Bahar ÖztürkAltan, MehmetSönmez, Osman FuatCakatay, UfukUzun, HafizeSimsek, Gönülhttps://hdl.handle.net/20.500.12900/3172024-02-13T08:15:48Z2023-01-01T00:00:00ZThe Role of Glycogen Synthase Kinase-3β in the Zinc-Mediated Neuroprotective Effect of Metformin in Rats with Glutamate Neurotoxicity
Oruc, Aykut; Oruc, Kadriye Yagmur; Yanar, Karolin; Mengi, Murat; Caglar, Aysel; Kurt, Bahar Öztürk; Altan, Mehmet; Sönmez, Osman Fuat; Cakatay, Ufuk; Uzun, Hafize; Simsek, Gönül
Metformin has been suggested to have protective effects on the central nervous system, but the mechanism is unknown. The similarity between the effects of metformin and the inhibition of glycogen synthase kinase (GSK)-3β suggests that metformin may inhibit GSK-3β. In addition, zinc is an important element that inhibits GSK-3β by phosphorylation. In this study, we investigated whether the effects of metformin on neuroprotection and neuronal survival were mediated by zinc-dependent inhibition of GSK-3β in rats with glutamate-induced neurotoxicity. Forty adult male rats were divided into 5 groups: control, glutamate, metformin + glutamate, zinc deficiency + glutamate, and zinc deficiency + metformin + glutamate. Zinc deficiency was induced with a zinc-poor pellet. Metformin was orally administered for 35 days. D-glutamic acid was intraperitoneally administered on the 35th day. On the 38th day, neurodegeneration was examined histopathologically, and the effects on neuronal protection and survival were evaluated via intracellular S-100β immunohistochemical staining. The findings were examined in relation to nonphosphorylated (active) GSK-3β levels and oxidative stress parameters in brain tissue and blood. Neurodegeneration was increased (p < 0.05) in rats fed a zinc-deficient diet. Active GSK-3β levels were increased in groups with neurodegeneration (p < 0.01). Decreased neurodegeneration, increased neuronal survival (p < 0.01), decreased active GSK-3β (p < 0.01) levels and oxidative stress parameters, and increased antioxidant parameters were observed in groups treated with metformin (p < 0.01). Metformin had fewer protective effects on rats fed a zinc-deficient diet. Metformin may exert neuroprotective effects and increase S-100β-mediated neuronal survival by zinc-dependent inhibition of GSK-3β during glutamate neurotoxicity.
2023-01-01T00:00:00ZNeutralization of Wild-Type and Alpha SARS-CoV-2 Variant by CoronaVac® Vaccine and Natural Infection- Induced AntibodiesÖzkaya, EsraYazıcı, MerveBaran, IrmakÇetin, Nesibe SelmaTosun, İlknurBuruk, Celal KurtuluşKaklikkaya, NeşeAydın, FarukDoymaz, Mehmet Ziyahttps://hdl.handle.net/20.500.12900/3082024-01-12T06:31:47Z2023-01-01T00:00:00ZNeutralization of Wild-Type and Alpha SARS-CoV-2 Variant by CoronaVac® Vaccine and Natural Infection- Induced Antibodies
Özkaya, Esra; Yazıcı, Merve; Baran, Irmak; Çetin, Nesibe Selma; Tosun, İlknur; Buruk, Celal Kurtuluş; Kaklikkaya, Neşe; Aydın, Faruk; Doymaz, Mehmet Ziya
One of the immune responses desired to be achieved by SARS-CoV-2 vaccination is to create neutralizing antibodies (nAbs),
thus preventing the development and spread of infection. The aim of this study was to investigate the seropositivity rate,
anti-spike antibody levels, and neutralizing capacity of these antibodies against wild type (WT) and alpha variants in serum
samples of individuals who had been naturally infected or vaccinated with CoronaVac®. Total anti-spike antibody levels
were determined in all samples. Neutralization assays were performed by the reduction of the cytopathic efect in Vero-E6
cells with infectious WT and alpha SARS-CoV-2 variants.
Although both naturally infected and vaccinated individuals were all seropositive for antispike antibodies, 84.8% of the vaccinated group, and 89.3% of the naturally infected group had detectable nAbs. The nAbs titers were signifcantly higher in
the naturally infected group for both WT and alfa variant of the virus as compared to the vaccinated individuals.
In this study, it was observed that all individuals became seropositive six weeks after exposure to the vaccine or the virus.
Moreover, naturally infected individuals had higher levels of nAbs than those vaccinated. The presence of nAbs against
the alpha variant in both naturally infected and vaccinated individuals suggests that these antibodies may also be protective
against infections, which may be caused by other variants, such as delta and omicron.
2023-01-01T00:00:00Z