Diş Hekimliği Fakültesihttps://hdl.handle.net/20.500.12900/112024-03-29T01:33:36Z2024-03-29T01:33:36ZIn vitro evaluation of the cytotoxic effects of alkasite restorative material on human dental pulp stem cellsElgun, TugbaCapan, Belen SirinogluDuman, CananOrdueri, Nazli Ece Gungorhttps://hdl.handle.net/20.500.12900/3202024-02-29T12:10:10Z2023-01-01T00:00:00ZIn vitro evaluation of the cytotoxic effects of alkasite restorative material on human dental pulp stem cells
Elgun, Tugba; Capan, Belen Sirinoglu; Duman, Canan; Ordueri, Nazli Ece Gungor
Aim: The clinical suitability of restorative dental materials is determined by evaluating their biocompatibility, and physical and chemical properties. The present
study examined the cytotoxic effects of three different dental restorative materials on human dental pulp stem cells (DPSCs).
Material and Methods: In this study, composite, high-viscosity glass ionomer cement (HVGIC), and an alkasite were used. In total, 12 samples of each material
were prepared for cytotoxicity assays. Cytotoxic effects were determined by considering biomaterial releases. Cell viability and proliferation were observed and
analyzed at intervals of 24 and 72 hours using both the methyl-thiazole-diphenyl-tetrazolium (MTT) and xCELLigence cytotoxicity assays. Data were calculated
using the RTCA-DP integrated software of the xCELLigence system and the GraphPad Prism 9.1.1 program. Data from the proliferation experiments were
statistically evaluated using the Two-way ANOVA test.
Results: Alkasite exhibited the highest cytotoxicity, whereas HVGIC and composite did not exhibit any significant difference compared with the control. There
was no difference between the two time points in the cytotoxicity of composite and alkasite in the MTT assay. However, the cytotoxicity of HVGIC was higher
at 72-hours than at 24-hours. Similar results were obtained with both assays. Although alkasite exhibited higher cytotoxicity than composite and HVGIC, all
materials exerted slightly cytotoxic effects (60%–90% cell viability) on DPSCs.
Discussion: Considering its aesthetic, and mechanical properties, alkasite can be clinically preferred instead of other materials in cavities that are not close
to the dental pulp.
2023-01-01T00:00:00ZWhat do TikTok videos offer us about dental implants treatment?Paksoy, TuğçeSen, Seval CeylanUstaoğlu, GülbaharBulut, Duygu Gollerhttps://hdl.handle.net/20.500.12900/3052024-01-09T13:23:47Z2023-01-01T00:00:00ZWhat do TikTok videos offer us about dental implants treatment?
Paksoy, Tuğçe; Sen, Seval Ceylan; Ustaoğlu, Gülbahar; Bulut, Duygu Goller
Objective: To evaluate the quality, source, popularity, visibility and reliability of Tiktok videos on dental implants. Materials and Methods: A Tiktok search for dental implants was performed English language setting. Search hashtags were determined as "#dentalimplants," "#dentalimplantsurgery," "#dentalimplantstreatment," and "#implantdentistry." 148 of the 300 videos watched were included the study. Two periodontologists scored the videos for quality, reliability, utility, visibility and popularity. Videos' quality was evaluated using the Global Quality Scale (GQS) and the DISCERN tools, by categorizing them through quality of communication, duration, likes and dislikes, views, source and video type of each video were recorded. Results: There was significant positive correlation between "GQS" and "Total DISCERN" (Rho: 0.636) and "Duration in seconds" (Rho: 0.343) (p < 0.05). For Total DISCERN, averages of the 31-45 seconds (p=0.010) and 46 seconds and above (p=.018) groups were higher than the averages of the 0-15 seconds group and average of the Educational group was higher than the average of the Testimonial, Product Advertisement and Entertainment groups (p=0.001, p=0.033 and p=0.041). Healthcare professionals mostly upload GQS 2 score videos and Hospital/Universities mostly upload GQS 2 and 3 score videos (p < 0.05). Testimonial videos mostly receive GQS2 score videos and the 'Videos rich in supplementary visuals' quality also receives the most GQS4 score videos (p < 0.05). Conclusions: The results of this study demonstrate that TikTok videos provide low to moderate quality information about dental implants and that TikTok may not provide reliable information about dental implants.
2023-01-01T00:00:00ZIs Online Video a Suitable Source to Obtain Sufficient and Useful Information About Peri-Implantitis?Bulut, Duygu GollerPaksoy, TuğçeUstaoğlu, Gülbaharhttps://hdl.handle.net/20.500.12900/3022024-01-09T06:50:20Z2023-01-01T00:00:00ZIs Online Video a Suitable Source to Obtain Sufficient and Useful Information About Peri-Implantitis?
Bulut, Duygu Goller; Paksoy, Tuğçe; Ustaoğlu, Gülbahar
Purpose: Recently, with the increase in cases of peri-implantitis, the amount of data patients can receive via social media about the condition has increased. The study presented here aims to examine what online videos (YouTubeTM, Google LLC, San Bruno, California) offer patients about peri-implantitis and to evaluate the quality of the information presented.Materials and Methods: In this cross-sectional study, a systematic search was conducted into online videos containing information about peri-implantitis using the keyword "peri-implantitis" by an experienced periodontologist. The source of videos, video type, duration, number of days since upload, number of views, comments, likes, dislikes, and interaction index of each video were recorded. Videos were scored according to content; Score 0 (low content): videos with no description of clinical presentations, risk factors, or management options; Score 1 (moderate content): videos that described 1 clinical presentation and 1 etiologic factor and did not describe management options; Score 2 (high content): videos with the description of at least 2 clinical presentations, 2 etiologic factors, and 1 management option. The quality of each video was evaluated using the Global Quality Scale and DISCERN. Data were analyzed using the Kruskal-Wallis test, Spearman's test, and multinomial logistic regression analysis. Intraobserver agreement was calculated as the intraclass correlation coefficient.Results: The first 200 videos were screened and, after exclusions, 103 videos were included for further analysis. United States-based videos were the majority (14 videos), 46.6% of the videos were uploaded by healthcare professionals, and 99% were in the educational videos category. Video content score 2 videos have higher mean values with 4,871.945 views, 6.002 comments, 50.729 likes, 7.751 viewing rate, and 0.023 interaction index than score 0 videos (P < .05). Statistically significant positive correlations were found between the video content score, Global Quality Scale, DISCERN, and the data showing the popularity and visibility of the videos (P < .01).Conclusions: Overall, half of the top-ranked online videos are valuable sources of information about peri-implantitis. Videos with high content are more popular and are of greater quality than videos with low content.(c) 2022 American Association of Oral and Maxillofacial SurgeonsJ Oral Maxillofac Surg 81:56-64, 2023
2023-01-01T00:00:00ZCould Long Non-Coding RNA MEG3 and PTENP1 Interact with miR-21 in the Pathogenesis of Non-Alcoholic Fatty Liver Disease?Erdem, Mustafa GencoÜnlü, ÖzgeDemirci, Mehmethttps://hdl.handle.net/20.500.12900/2822024-01-05T13:19:08Z2023-01-01T00:00:00ZCould Long Non-Coding RNA MEG3 and PTENP1 Interact with miR-21 in the Pathogenesis of Non-Alcoholic Fatty Liver Disease?
Erdem, Mustafa Genco; Ünlü, Özge; Demirci, Mehmet
NAFLD is the most common cause of chronic liver disease worldwide. The miRNAs and lncRNAs are important endogenous ncRNAs families that can regulate molecular mechanisms. The aim of this study was to analyze the miRNA and lncRNA expression profiles in serum samples of NAFLD patients with different types of hepatosteatosis compared to healthy controls by the qPCR method. A total of180 NAFLD patients and 60 healthy controls were included. miRCURY LNA miRNA miRNome PCR human panel I + II kit and LncProfiler qPCR Array Kit were used to detect miRNA and lncRNA expression, respectively. DIANA miRPath and DIANA-lncBase web servers were used for interaction analysis. As a result, 75 miRNA and 24 lncRNA expression changes were determined. For miRNAs and lncRNAs, 30 and 5 were downregulated and 45 and 19 were upregulated, respectively. hsa-miR-21 was upregulated 2-fold whereas miR-197 was downregulated 0.25-fold. Among lncRNAs, NEAT1 was upregulated 2.9-fold while lncRNA MEG3 was downregulated 0.41-fold. A weak correlation was found between hsa-miR-122 and lncRNA MALAT1. As a conclusion, it is clear that lncRNA-miRNA interaction is involved in the molecular mechanisms of the emergence of NAFLD. The lncRNAs MEG3 and PTENP1 interacted with hsa-miR-21. It was thought that this interaction should be investigated as a biomarker for the development of NAFLD.
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