Dahili Tıp Bilimleri Bölümü Koleksiyonuhttps://hdl.handle.net/20.500.12900/762024-03-28T12:58:14Z2024-03-28T12:58:14ZAssessment of subtle cardiac dysfunction induced by premature ventricular contraction using two-dimensional strain echocardiography and the effects of successful ablationDoğan, ZekiErden, Emine CakcakErden, İsmailBektaşoğlu, Gökhanhttps://hdl.handle.net/20.500.12900/3142024-03-07T11:14:28Z2024-01-01T00:00:00ZAssessment of subtle cardiac dysfunction induced by premature ventricular contraction using two-dimensional strain echocardiography and the effects of successful ablation
Doğan, Zeki; Erden, Emine Cakcak; Erden, İsmail; Bektaşoğlu, Gökhan
Introduction and objectives: We aimed to assess the effects of successful ablation on impaired left ventricular global longitudinal strain (LV-GLS) in patients with frequent premature ventricular contractions (PVCs). We also evaluated the potential risk factors of impaired LV-GLS.Methods: Thirty-six consecutive patients without any structural heart disease, who were treated with radiofrequency (RF) ablation due to frequent PVCs, were included in the study. All patients were evaluated with standard transthoracic and two-dimensional speckle tracking echocardiography.Results: Mean LV-GLS before ablation was 17.3 +/- 3.7 and 20.5 +/- 2.6 after ablation; the difference was statistically significant (p<0.01). Patients were categorized into two groups: those with LV-GLS value >-16% and those <= 16%. Low PVC E flow/post-PVC E flow and PVC SV/post-PVC SV ratios were associated with impaired LV-GLS.Conclusion: In symptomatic patients with frequent PVCs and normal left ventricular ejection fraction, we observed significant improvement in LV-GLS value following successful RF ablation. Patients with impaired LV-GLS more often display non-ejecting PVCs and post-extrasystolic potentiation (PEP) compared to patients with normal LV-GLS.(c) 2023 Sociedade Portuguesa de Cardiologia. Published by Elsevier Espan similar to a, S.L.U. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/bync-nd/4.0/).
2024-01-01T00:00:00ZAlterations in expression of phosphatase and tensin homologous (PTEN) gene analyzed in the bronchial lavage samples of non-small cell lung cancer patientsSahin, HilalKuru, DilhanYılmaz, NailAtahan, ErsanDeviren, AyhanTuncdemir, Matemhttps://hdl.handle.net/20.500.12900/3132024-03-07T06:56:08Z2024-01-01T00:00:00ZAlterations in expression of phosphatase and tensin homologous (PTEN) gene analyzed in the bronchial lavage samples of non-small cell lung cancer patients
Sahin, Hilal; Kuru, Dilhan; Yılmaz, Nail; Atahan, Ersan; Deviren, Ayhan; Tuncdemir, Matem
Aim: In this study, we aimed to analyze the expression of the Phosphatase and Tensin Homologous gene (PTEN) by interphase-fluorescence in situ-hybridization (iFISH), and localization and immunoreactivity of PTEN by immunocytochemistry method in bronchoalveolar lavage (BAL) fluid of patients with non-small cell lung cancer (NSCLC).Material and Methods: BAL samples collected from 30 patients diagnosed with NSCLC and from 10 control subjects without any malignancy were included. iFISH was performed with a 10q23.3 PTEN DNA dual color FISH probe, analyzing multiple cells. Immunocytochemistry was based on the Streptavidin-Biotin-Peroxidase method and PTEN levels were semi-quantitatively scored.Results: The percentage of cells with 2G/2R was 86.8% which was significantly lower than that of the control group (p<0.0001). Loss of the PTEN gene was 6.43% among all patients. The percentage of cells with monoallelic deletion (p=0.0246) and atypical signal (p=0.0001) and the percentage of monosomic cells (p=0.0001) were significantly higher in patients compared to controls. PTEN immunopositivity was significantly increased in patients compared to controls (p<0.0001).Discussion: Increased abnormal signals and mutational cells in BAL samples of NSCLC patients suggest that the investigation of the PTEN gene in BAL by using iFISH may guide clinicians in differential diagnosis and therapeutic management of NSCLC. Being faster, easier and cost-effective in routine testing, the immunocytochemistry method can also be combined as a diagnostic tool with molecular methods.
2024-01-01T00:00:00ZEvaluation of Clinical and Immunological Alterations Associated with ICF SyndromeBilgic Eltan, SevgiNain, ErcanCatak, Mehmet CihangirEzen, EgeSefer, Asena PinarKarimi, NastaranKiykim, AycaKolukisa, BurcuBaser, DilekBulutoglu, AlperKasap, NurhanYorgun Altunbaş, MelekYalçın Güngören, EzgiKendir Demirkol, YaseminKutlug, SeyhanHancioglu, GoncaDilek, FatihYıldıran, AlişanOzen, AhmetKarakoc-Aydiner, ElifErman, BatuBaris, Safahttps://hdl.handle.net/20.500.12900/3122024-03-07T06:51:32Z2024-01-01T00:00:00ZEvaluation of Clinical and Immunological Alterations Associated with ICF Syndrome
Bilgic Eltan, Sevgi; Nain, Ercan; Catak, Mehmet Cihangir; Ezen, Ege; Sefer, Asena Pinar; Karimi, Nastaran; Kiykim, Ayca; Kolukisa, Burcu; Baser, Dilek; Bulutoglu, Alper; Kasap, Nurhan; Yorgun Altunbaş, Melek; Yalçın Güngören, Ezgi; Kendir Demirkol, Yasemin; Kutlug, Seyhan; Hancioglu, Gonca; Dilek, Fatih; Yıldıran, Alişan; Ozen, Ahmet; Karakoc-Aydiner, Elif; Erman, Batu; Baris, Safa
Purpose: Immunodeficiency with centromeric instability and facial anomalies (ICF) syndrome is a rare autosomal recessive combined immunodeficiency. The detailed immune responses are not explored widely. We investigated known and novel immune alterations in lymphocyte subpopulations and their association with clinical symptoms in a well-defined ICF cohort. Methods: We recruited the clinical findings from twelve ICF1 and ICF2 patients. We performed detailed immunological evaluation, including lymphocyte subset analyses, upregulation, and proliferation of T cells. We also determined the frequency of circulating T follicular helper (cTFH) and regulatory T (Treg) cells and their subtypes by flow cytometry. Results: There were ten ICF1 and two ICF2 patients. We identified two novel homozygous missense mutations in the ZBTB24 gene. Respiratory tract infections were the most common recurrent infections among the patients. Gastrointestinal system (GIS) involvements were observed in seven patients. All patients received intravenous immunoglobulin replacement therapy and antibacterial prophylaxis; two died during the follow-up period. Immunologically, CD4+ T-cell counts, percentages of recent thymic emigrant T cells, and naive CD4+ T decreased in two, five, and four patients, respectively. Impaired T-cell proliferation and reduced CD25 upregulation were detected in all patients. These changes were more prominent in CD8+ T cells. GIS involvements negatively correlated with CD3+ T-, CD3+CD4+ T-, CD16+CD56+ NK-cell counts, and CD4+/CD8+ T-cell ratios. Further, we observed expanded cTFH cells and reduced Treg and follicular regulatory T cells with a skewing to a TH2-like phenotype in all tested subpopulations. Conclusion: The ICF syndrome encompasses various manifestations affecting multiple end organs. Perturbed T-cell responses with increased cTFH and decreased Treg cells may provide further insight into the immune aberrations observed in ICF syndrome.
2024-01-01T00:00:00ZAssessment of sexual dysfunction, the quality of life and difficulties in broaching sexual issues in patients with chronic low back pain by using cluster analysisCokar, DilekCelik, YusufAyla, Ozge OzdemirKavadar, GulisMurat, SadiyeOzgonenel, Leventhttps://hdl.handle.net/20.500.12900/3112024-02-05T12:11:49Z2024-01-01T00:00:00ZAssessment of sexual dysfunction, the quality of life and difficulties in broaching sexual issues in patients with chronic low back pain by using cluster analysis
Cokar, Dilek; Celik, Yusuf; Ayla, Ozge Ozdemir; Kavadar, Gulis; Murat, Sadiye; Ozgonenel, Levent
The aim of this study is to investigate the physical and psychosocial problems and the clinical and sociodemographic factors affecting sexual life in patients with nonspecific chronic low back pain (CLBP), thus drawing attention to sexual disability and patients' concerns in the conservative management of the disease. A total of 140 patients were included in the study. The assessment of disease characteristics, information about sexual life and related factors were evaluated by a self-constructed questionnaire. Physical Activity Questionnaire (IPAQ), Tampa Scale of Kinesiophobia (TSK) and Beck Depression Inventory (BDI) were used to assess functional disability, pain-related anxiety, physical activity level, fear of movement and depression severity, respectively. Hierarchical cluster analysis was used to discover relationships among variables. Based on the dendrogram, the intensity of CLBP, the impact of CLBP on sexual life, decrease in libido, sexual satisfaction, frequency of intercourse, painful sexual behavior and questionnaire results (BDI, TSK, ODI, PCS) were in the same cluster. Consistent with this, positive correlations were found between BDI and PCS (p < 0.001, r = 0.481), BDI and TSK (p < 0.001, r = 0.319), and BDI and ODI (p < 0.001, r = 0.408). The other main clusters included demographic and clinical characteristics, ODI and IPAQ scores. The sexual lives of patients with CLBP are affected physically and psychosocially. In clinical practice, it is important to counsel sexual disability patients because of their concerns and hesitations.
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